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Welcome to the home page of the Biological Crystallography Facility at the NSRRC (BioNSRRC). Biological Crystallography is a technique that can determinate the 3D molecular structures of biological macromolecules from their single crystals. The goal of BioNSRRC is to provide users with a sophisticated research facility, including a full complement of instrumentation, software, and support for high-throughput data collection and processing. We also provide professional discussions and substantial user support; therefore both experienced and non-experienced users are all welcome to use this facility. Worldwide researchers can access to this facility through the NSRRC proposal submitting procedure.

BioNSRRC Beamline
  Location
TLS, NSRRC
TLS, NSRRC
TLS, NSRRC
TPS, NSRRC
  X-ray Source
Wiggler on
1.5GeV TLS
Wiggler on
1.5GeV TLS
Wiggler on
1.5GeV TLS
Undulator on
3GeV TPS
  General User Time
80%
80%
80%
80%
  Status
Operational
Operational
Operational
Operational
  Experiment
Mono
MAD
MAD
MAD/Microbeam
  Wavelength (Å)
0.975
1.77-0.69
2.21-0.8
2.26-0.62
  Energy Range (keV)
12.7
7-18
5.6-15.5
5.7-20.0
  Aperture Size (μmΦ)
200
200/150/100/75/50
200/150/100/75/50
50/30/20/10/5
  Flux After Aperture(photons/sec)
3.5x1010
12/7/3/2/0.8x1010
14/9/4/2/1.1x10 10
270/140/63/38/8.2x1010
  Flux Density (photons/sec/μm2)
3.3x10 6
3.3–4.8x10 6
5.7–7.5x10 6
2.6–3.3x10 9
  Time to Henderson Limit (sec)
12121
8333–12121
5333–7018
12.1–15.4
  Detector 
Q315r
Q315r
MX300HE
MX300HS
  Detector Size (mm2)
315x315
315x315
300x300
300x300
  Xtal-Detector (mm)
110-900
130-800
100-850
100-900
  Throughput (images/min)
20
20
20
900
  Normal Rotation Method
Yes
Yes
Yes
Yes
  Grid Scan Search
No
No
No
Yes
  Helical Scan Method
No
No
No
Yes
  Multi-position Method
No
No
No
Yes
SAM
SAM
SAM
SAM
Yes
Yes
Yes
Yes

 Historical Events

   The first de-novo structure solved at BioNSRRC on 2001

   The first protein structure selected as the Research Highlights of 2008 by NIH, USA

   The first membrane protein structure solved at BioNSRRC on 2009

   Human topoisomerase IIbeta in complex with DNA and etoposide solved at BioNSRRC on 2011

   Membrane embedded H1-translocating pyrophosphatase solved at BioNSRRC on 2012

 Funding Agencies

   Ministry of Science and Technology, Taiwan, ROC

   National Core Facility Program for Biotechnology

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