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Welcome to the home page of the Biological Crystallography Facility at the NSRRC (BioNSRRC). Biological Crystallography is a technique that can determinate the 3D molecular structures of biological macromolecules from their single crystals. The goal of BioNSRRC is to provide users with a sophisticated research facility, including a full complement of instrumentation, software, and support for high-throughput data collection and processing. We also provide professional discussions and substantial user support; therefore both experienced and non-experienced users are all welcome to use this facility. Worldwide researchers can access to this facility through the NSRRC proposal submitting procedure.

BioNSRRC Beamline	BL13C1	BL13B1	BL15A1	TPS 05A
Location	TLS, NSRRC	TLS, NSRRC	TLS, NSRRC	TPS, NSRRC
X-ray Source	Wiggler on 1.5GeV TLS	Wiggler on 1.5GeV TLS	Wiggler on 1.5GeV TLS	Undulator on 3GeV TPS
General User Time	80%	90%	80%	75%
Status	Operational	Operational	Operational	Operational
Experiment	Mono	MAD	MAD	MAD/Microbeam
Wavelength (Å)	0.975	1.77-0.69	2.21-0.8	2.26-0.62
Energy Range (keV)	12.7	7-18	5.6-15.5	5.7-20.0
Aperture Size (μmΦ)	200	200/150/100/75/50	200/150/100/75/50	50/30/20/10/5
Flux After Aperture(photons/sec)	3.5x10¹⁰	12/7/3/2/0.8x10¹⁰	14/9/4/2/1.1x10 ¹⁰	270/140/63/38/8.2x10¹⁰
Flux Density (photons/sec/μm²)	3.3x10 ⁶	3.3–4.8x10 ⁶	5.7–7.5x10 ⁶	2.6–3.3x10 ⁹
Time to Henderson Limit (sec)	12121	8333–12121	5333–7018	12.1–15.4
Detector	Q315r	Q315r	MX300HE	MX300HS
Detector Size (mm²)	315x315	315x315	300x300	300x300
Xtal-Detector (mm)	110-900	130-800	100-850	100-900
Throughput (images/min)	20	20	20	900
Normal Rotation Method	Yes	Yes	Yes	Yes
Grid Scan Search	No	No	No	Yes
Helical Scan Method	No	No	No	Yes
Multi-position Method	No	No	No	Yes
Sample Changer	SAM	SAM	SAM	SAM
Remote Access	Yes	Yes	Yes	Yes

Historical Events

The first de-novo structure solved at BioNSRRC on 2001

The first protein structure selected as the Research Highlights of 2008 by NIH, USA

The first membrane protein structure solved at BioNSRRC on 2009

Human topoisomerase IIbeta in complex with DNA and etoposide solved at BioNSRRC on 2011

Membrane embedded H1-translocating pyrophosphatase solved at BioNSRRC on 2012

Funding Agencies

Ministry of Science and Technology, Taiwan, ROC

National Core Facility Program for Biotechnology