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Welcome to the home page of the Biological Crystallography Facility at the NSRRC (BioNSRRC). Biological Crystallography is a technique that can determinate the 3D molecular structures of biological macromolecules from their single crystals. The goal of BioNSRRC is to provide users with a sophisticated research facility, including a full complement of instrumentation, software, and support for high-throughput data collection and processing. We also provide professional discussions and substantial user support; therefore both experienced and non-experienced users are all welcome to use this facility. Worldwide researchers can access to this facility through the NSRRC proposal submitting procedure.

BioNSRRC Beamline	BL15A1	TPS 05A	TPS 07A
Location	TLS, NSRRC	TPS, NSRRC	TPS, NSRRC
X-ray Source	Wiggler on 1.5GeV TLS	Undulator on 3GeV TPS	Undulator on 3GeV TPS
General User Time	80%	75%	75%
Status	Operational	Operational	Operational
Experiment	MAD	MAD/Microbeam	MAD/Microfocus
Wavelength (Å)	2.21-0.8	2.26-0.62	2.06-0.62
Energy Range (keV)	5.6–15.5	5.7–20.0	6–20.0
Aperture Size (μmΦ)	200/150/100/75/50	200/150/100/75/60/50/40/30/20/10	100/90/80/70/60/50/40/30/20/10/5/2
Flux After Aperture(photons/sec)	14/9/4/2/1.1x10¹⁰	570/380/170/120/100/44/32/19/15/4x10¹⁰	820/870/650/340/170/8.6x10¹¹
Flux Density (photons/sec/μm²)	5.7–7.5x10⁶	1.8–5x10⁸	9.4–1600x10⁸
Time to Henderson Limit (sec)	5333–7018	12.1–15.4	0.24–42.6
Detector	MX300HE	Eiger2 X 9M	Eiger2 X 16M
Detector Size (mm²)	300x300	233.1x244.7	311.1x327.2
Xtal-Detector (mm)	100-850	110-900	140-900
Throughput (images/min)	20	12000 (200Hz)	7200 (120Hz)
Normal Rotation Method	Yes	Yes	Yes
Grid Scan Search	No	Yes	Yes
Helical Scan Method	No	Yes	Yes
Multi-position Method	No	Yes	Yes
Sample Changer	SAM	ISARA	No
Remote Access	Yes	Yes	No

Historical Events

The first de-novo structure solved at BioNSRRC on 2001

The first protein structure selected as the Research Highlights of 2008 by NIH, USA

The first membrane protein structure solved at BioNSRRC on 2009

Human topoisomerase IIbeta in complex with DNA and etoposide solved at BioNSRRC on 2011

Membrane embedded H1-translocating pyrophosphatase solved at BioNSRRC on 2012

Funding Agencies

National Science and Technology Council, Taiwan, ROC

National Core Facility for Biotechnology