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Beamline

The BioNSRRC has currently four beamlines (BL13C1, BL13B1, BL15A1, and TPS 05A) in operation. BL13B1, BL15A1, and TPS 05A are capable of performing MAD experiments, as well as standard monochromatic crystallography. BL13C1 is designed to operate at fixed energy above the Se K edge. All four beamlines also serve as the high-throughput Synchrotron Protein Crystallography Facility (SPXF) of the National Core Facility Program for Biotechnology (NCFPB) to provide the NCFPB users with reliable, rapid and fully automated X-ray beamlines.

BioNSRRC Beamline	BL13C1	BL13B1	BL15A1	TPS 05A
Location	TLS, NSRRC	TLS, NSRRC	TLS, NSRRC	TPS, NSRRC
X-ray Source	Wiggler on 1.5GeV TLS	Wiggler on 1.5GeV TLS	Wiggler on 1.5GeV TLS	Undulator on 3GeV TPS
General User Time	80%	90%	80%	75%
Status	Operational	Operational	Operational	Operational
Experiment	Mono	MAD	MAD	MAD/Microbeam
Wavelength (Å)	0.975	1.77-0.69	2.21-0.8	2.26-0.62
Energy Range (keV)	12.7	7-18	5.6-15.5	5.7-20.0
Aperture Size (μmΦ)	200	200/150/100/75/50	200/150/100/75/50	50/30/20/10/5
Flux After Aperture(photons/sec)	3.5x10¹⁰	12/7/3/2/0.8x10¹⁰	14/9/4/2/1.1x10 ¹⁰	270/140/63/38/8.2x10¹⁰
Flux Density (photons/sec/μm²)	3.3x10 ⁶	3.3–4.8x10 ⁶	5.7–7.5x10 ⁶	2.6–3.3x10 ⁹
Time to Henderson Limit (sec)	12121	8333–12121	5333–7018	12.1–15.4
Detector	Q315r	Q315r	MX300HE	MX300HS
Detector Size (mm²)	315x315	315x315	300x300	300x300
Xtal-Detector (mm)	110-900	130-800	100-850	100-900
Throughput (images/min)	20	20	20	900
Normal Rotation Method	Yes	Yes	Yes	Yes
Grid Scan Search	No	No	No	Yes
Helical Scan Method	No	No	No	Yes
Multi-position Method	No	No	No	Yes
Sample Changer	SAM	SAM	SAM	SAM
Remote Access	Yes	Yes	Yes	Yes